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Phylogenetic Analyses of Teleki Grapevine Rootstocks Using Three Chloroplast DNA Markers

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Abstract

Teleki rootstocks are used in grapevine-producing countries all over the world. They represent one of the largest groups of available rootstocks but their origin is still in dispute although they have been regarded as Vitis berlandieri × V. riparia hybrids. To investigate their possible origin, we amplified and sequenced three chloroplast regions, two non-coding spacers (trnL-F, trnS-G) and the trnL group I intron in a core collection of Teleki rootstocks representing widespread accessions and related wild North American grape species (V. berlandieri, V. riparia and V. rupestris). Concatenated sequence data coupled with microstructural changes discovered in the chloroplast regions provided data to trace the maternal ancestry of the Teleki lines. All chloroplast regions showed both nucleotide and length variation. Length mutations in the non-coding regions represented mostly simple sequence repeats of poly-A and -T stretches. These indel characters exhibited additional diversity comparable with the nucleotide diversity and increased resolution of the phylogenetic trees. We found that a group of Teleki accessions position together with the wild grape species V. riparia. Another group of Teleki rootstocks formed a sister group to the other North American species V. berlandieri. These clades had moderate support values, and they do not share ancestry with other accessions of Teleki rootstocks resolved with high support value in the V. riparia clade. It seems that Teleki-Kober 5BB and 125 AA accessions might have a V. berlandieri maternal background. We also found great differences within putative clones of Teleki 5C and Teleki-Kober 5BB suggesting that the selection of these accessions was performed on heterogenous or mislabeled plant material collectively maintained under these names.

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Acknowledgements

Thanks are due to Kinga Klára Mátyás for laboratory assistance, Nándorné Nagy and Tamás Lönhard for field collections. We also thank Ildikó Varga for her help preparing the figures. This work was supported by the Hungarian Scientific Research Fund (project no. K73708). Peter Poczai gratefully acknowledges support from the Mare Curie Fellowship Grant (PIEF-GA-2011-300186) under the seventh framework program of the European Union.

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Authors and Affiliations

  1. Plant Biology, Department of Biosciences, University of Helsinki, PO Box 65, 00014, Helsinki, Finland

    Péter Poczai & Jaakko Hyvönen

  2. Department of Plant Science and Biotechnology, Georgikon Faculty, University of Pannonia, Festetics 7, 8360, Keszthely, Hungary

    János Taller

  3. Research Institute for Viticulture and Oenology, Centre of Agricultural Sciences, University of Pannonia, Római út 181, 8261, Badacsonytomaj, Hungary

    Gizella Jahnke

  4. Department of Horticulture, Georgikon Faculty, University of Pannonia, Festetics 7, 8360, Keszthely, Hungary

    László Kocsis

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  1. Péter Poczai
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Correspondence to Péter Poczai.

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Supplementary Table 1

Information on the origin, genetic background of accessions and GenBank accession numbers for the sequenced chloroplast regions used in this study. *The region includes the trnL intron and the trnL-trnF spacer region under one accession number. (DOCX 30 kb)

Supplementary Table 2

Concatenated MUSCLE sequence alignment. (DOC 2453 kb)

Supplementary Table 3

Concatenated GBlocks sequence alignment. (DOC 1936 kb)

Supplementary Table 4

List of microstructural changes found in the trnL-trnF and trnS-trnG chloroplast regions (DOCX 20 kb)

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Poczai, P., Hyvönen, J., Taller, J. et al. Phylogenetic Analyses of Teleki Grapevine Rootstocks Using Three Chloroplast DNA Markers. Plant Mol Biol Rep 31, 371–386 (2013). https://doi.org/10.1007/s11105-012-0512-9

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  • DOI: https://doi.org/10.1007/s11105-012-0512-9

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